Circulating cell-free DNA (cfDNA) in blood holds great potential for cancer detection, as highlighted by a DNA methylation-based, cfDNA multi-cancer early detection test that is being prepared for prospective clinical investigation. 1
Though cfDNA is highly fragmented, the Zymo-Seq RRBS Library Kit has successfully used plasma cfDNA as the starting material to generate libraries for reduced representation bisulfite sequencing. This user-friendly kit allows researchers to:
- Generate libraries from as little as 10 ng of cfDNA
- Detect over 2 million unique CpG Sites (≥5X)
- Obtain reproducible methylation values
Read more about Zymo here.