Visualize Actin Structures with Phalloidin Derived Stains and Antibodies

Explore AAT Bioquest’s robust portfolio of F-actin staining probes that selectively targets and binds on F-actin filaments.

Fluorescent Phalloidin-iFluor Conjugates

  • Optimized for visualizing actin filaments in vitro
  • Compatible with live cells, formaldehyde-fixed samples, permeabilized tissue sections, cell cultures or cell-free experiments
  • Available in multi-colour wavelengths for multi-staining applications
  • Suitable for fluorescence microscopy, microplate and flow cytometry

Fluorescence image of HeLa cells stained with Phalloidin-iFlour 488 (left) and visualized using a fluorescence microscope equipped with a FITC filter set. Fluorescence image of HeLa cells stained with Phalloidin-iFlour 514 (middle) and visualized using a fluorescence microscope equipped with a TRITC filter set. Fluorescence image of HeLa cells stained with Phalloidin-iFlour 647 (right) and visualized using a fluorescence microscope equipped with a Cy5 filter set.

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Secondary Antibodies

HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by iFluor™ 488 goat anti-mouse IgG conjugate (Green, Left) or Alexa Fluor® 488 goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue).

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