BioSite Newsletter Q2 2018
"The season after winter and before summer, in which vegetation begins to appear, in the northern hemisphere from March to May and in the southern hemisphere from September to November."
Unfortunately, it seems that we have to wait somewhat longer than usual for Spring... So in the meantime, we are doing our very best to give you bright, interesting and uplifting news. Such as our new blog post, The Lowdown on Real-Time PCR – Part I, or the first and only LBC Control for Quantitative Automated Cell Counts from Streck and Reliable Assays to Measure Reactive Oxygen Species. You can read about all of this in the latest newsletter from Nordic BioSite.
Should you have any questions, don’t hesitate to contact us, we are by your side!
- Your Nordic BioSite Team
The Lowdown on Real-Time PCR – Part I
In case the name doesn't give it away, real-time PCR is a PCR application that monitors DNA amplification in real time. This means that amplification is monitored during the PCR reaction, and not at the end of the reaction as with end-point PCR, where PCR products are typically analyzed post-run on agarose gels.
This article is the first in a two-part series. In the next part we will cover the different quantification methods available, setup tips, primer design and quality control.
LBC Control for Quantitative Automated Cell Counts
Robust Assays for Reactive Oxygen Species (ROS)
Reactive oxygen species (ROS) are continually produced during metabolic processes, and ROS generation is normally counterbalanced by the action of antioxidant enzymes and other redox molecules. However, excess reactive oxygen species must be promptly eliminated from the cell by a variety of antioxidant defense mechanisms. Excess ROS can lead to cellular injury in the form of damaged DNA, lipids and proteins.