Useful monoclonal antibodies for the research of cancer pathology

Laminins are major extracellular matrix proteins containing over 15 members. Laminins are large hetero-trimers consisting of 5 alpha chains, 3 beta chains, and 3 gamma chains. Due to its structural varieties, it has been

challenging to detect each laminin isoform by conventional antibodies. Our supplier Funakoshi commercializes 4 unique antibodies, originally developed by Dr. Kaoru Miyazaki, Professor Emeritus of Yokohama City University in Japan. Our antibodies can separately recognize specific isoforms or structures of laminin.

Fig.1 Structure of Laminin 3A32, 3B32 and γ2pf fragment with epitopes of 3 clones (F7, BG5 andP2H).

 

Featured Products

 

BG5/F7 : For laminin alpha-3 chains

- Laminin alpha 3 chain, a major component of Laminin-332, has two splicing variants, alpha 3A and alpha 3B
- Clone BG5 is specific antibody for alpha 3A
- Clone F7 is the world first commercially available antibody for alpha 3B

P2H : For laminin gamma-2 chain

- Laminin gamma2, an only component of Laminin-332, is a marker gene of invasive cancer tissue
- Laminin gamma-2 chain is activated via proteolytical cleavage at N-terminal
- N-terminal proteolytic fragment of gamma-2, called γ2pf, is highly accumulated into cytosol under malignant cancer
- Clone P2H is the world first antibody for γ2pf

12D : For laminin-511

- There are two similar isoforms, laminin-511 and laminin-521 and it is difficult for conventional antibodies such as anti-alpha 5 chain to separate them.
- Clone 12D specifically react with trimeric structure of laminin-511, not react with each components
alpha 5, beta 1, gamma 1 and similar isoform laminin-511.   

Note: Laminin 511 is used as a culture substrate for ES and iPS cells.

 

Fig.2 Structure of Laminin-511

 

Description  Anti-Laminin alpha 3A Anti-Laminin alpha 3B Anti-Laminin gamma 2 N-terminal Fragment Anti-Laminin-511
Clone BG5 F7 P2H 12D
Specificity Laminin alpha 3A *1      
Host and Type Mouse Monoclonal
Subclass  IgG2a IgG1 IgG2a IgG2b
Reactivity  Human Human Human and Mouse Human
Form  Mouse Ascites (Unpurified)      
Application  ELISA, IHC, IP, WB    IHC (frozen & paraffin sextions), WB IHC (frozen), IP, WB (non-reducing) *2
Code  FDV-0024 FDV-0023  FDV-0025 FDV-0026
Size  100 uL
Storage -20℃ *3

*1 Clone BG5 only detects alpha 3A in IHC. However, this clone may react with alpha 3B in ELISA, IP and Western Blot.

*2 Clone 12D can be only used for IHC (Frozen) and under non-reducing condition for Western Blot.

*3 Non-preservative added. So these 4 clones can be degraded at 4C. We recommend -20C storage.

 

Data Examples

 

Fig.3 IHC image of skin cancer tissue in clone BG5 and F7

Left : Clone BG5, Right : Clone F7

Both clones detectedbasal membrane like structure around the tumor but F7 clone also detected

vascular basement membrane (arrow heads).

Fig.4 IHC image of tissues in clone P2H

Upper images : Normal mammary gland

Lower images: Breast cancer

P2H stains basement membranes surrouding mammary glands in normal tisue,

whereas in a breast cancer tissue cytoplasmic accumulation of γ2pf orits fragments

is detected.

 

Fig.5 IHC image ofbreast cancer tissue in clone 12D

A : Vascular basement membrane

B: Mammary gland basement membrane

 

C : Basement membrane-like structures surrounding tumor cells

Fig.6 Specificity of clone 12D against laminin isoforms

Seven recombinant human laminins were coated on a multi-well

plate and detected by clone 12D. The data shows clone 12D

specifically reacts with Laminin 511.

Citations and References

1. Kariya, Y., et al., J. Mol. Histol., 39, 435-446 (2008) (Clone BG5/F7)

2. Mori, T., et al., Cancer Sci., 102, 1095-1100 (2011) (Clone F7)

3. Miyazaki, K., et al., Cancer Sci., 107, 1909-1918 (2016) (Clone BG5, P2H)

4. Komiya, E., et al., Cancer Med., 3, 537-549 (2014) (Clone 12D)