LC3: The gold standard in autophagy detection
LC3 has been studied most extensively and is frequently used as an autophagy marker in mammals. Newly translated LC3 (proLC3) is immediately processed at the C-terminus in order to form LC3-I.
Autophagosomes fuse with lysosomes to form autolysosomes, and intra-autophagosomal components are degraded by lysosomal hydrolases. At the same time, LC3-II in autolysosomal lumen is degraded. Thus, lysosomal turnover of the autophagosomal marker LC3-II reflects starvation-induced autophagic activity, and detecting LC3 by immunoblotting or immunofluorescence has become a reliable method for monitoring autophagy and autophagy-related processes, including autophagic cell death.
In order to determine the levels of autophagy, anti-LC3 antibodies can be used for detection of endogenous LC3-II by immunoblotting, immunoprecipitation, and immunofluorescence.
|Cat. No.||Name||Species Reactivity||Applications|
|M152-3||Anti-LC3 (Human) Monoclonal Antibody||Human, Mouse, Rat Hamster||FCM, ICC, IP, WB|
|M186-3||Anti-LC3 (Human) Monoclonal Antibody||Human, Mouse, Rat Hamster||WB|
|M186-7||Anti-LC3 Monoclonal Antibody-HRP-DirecT||Human, Mouse, Rat Hamster||WB|
|PD014||Anti-LC3 (Rat) Polyclonal Antibody||Human, Mouse, Rat Hamster||WB|
|PM036||Anti-LC3 (Human) Polyclonal Antibody||Human, Mouse, Rat Hamster||FCM. ICC, IHC, IP, WB|
|CY-7055||CycLex® Total p62 ELISA Kit||ELISA|
|CY-7056||CycLex® Phospho-p62 Ser349 ELISA Kit||ELISA|
|CY-7057||CycLex® Phospho-p62 Ser403 ELISA Kit||ELISA|