Quantitatively Measure Allopregnanolone
Allopregnanolone is synthesized from progesterone which is converted into 5α-dihydroprogesterone by 5α-reductase type I, and 3α-hydroxysteroid oxidoreductase isoenzymes. This 5α-dihydro intermediate is then converted into allopregnanolone. 3α-hydroxysteroids such as allopregnanolone, do not interact with classical intracellular steroid receptors but bind stereoselectively and with high affinity to receptors for the major inhibitory neurotransmitter in brain, γ-amino-butyric acid (GABA).
Allopregnanolone may be involved in neuronal plasticity, learning and memory processes, aggression and epilepsy, and the modulation of the responses to stress, anxiety and depression. It aids neurogenesis and has been found to reverse neuron proliferative deficit and cognitive deficits in mouse models of Alzheimer’s disease. Allopregnanolone has also been shown to restore functionality in a mouse model of Parkinson’s Disease. In addition, it has been shown to improve behavioral problems in both post-traumatic stress disorder and schizophrenia. A recent study by Yao et. al., shows the importance of measuring allopregnanolone levels when evaluating patients with schizophrenia.
The DetectX® Allopregnanolone EIA and CLIA kits from Arbor Assays are designed to quantitatively measure allopregnanolone present in biological samples. This kit measures total allopregnanolone in extracted serum or plasma and fecal samples. An allopregnanolone stock solution is provided to generate a standard curve, standards or diluted samples are pipetted into microtiter plates, and an allopregnanolone-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of an antibody to allopregnanolone. After incubation the plate is washed and substrate is added. The substrate reacts with the bound allopregnanolone-peroxidase conjugate. For the EIA kits the plate is read at 450 nm, and the CLIA kits the light emission is read.
- Use: Easily Quantitate this Neurosteroid
- Sample: Extracted Serum and Plasma, Urine, Fecal Extracts, TCM
- Species: Species Independent
- Time to Answer: 2.5 Hours EIA / Overnight CLIA
- Samples/Kit: ≥ 38
- Stability: Liquid Reagents, 4°C Storage
- Selective: Low Cross Reactivity to Progesterone and Metabolites
Typical EIA & CLIA Standard Curves