Triculture Model for Alzheimer’s Disease

Triculture Model

In vitro models can be a helpful tool for recreating the conditions of Alzheimer’s disease (AD). However, many systems fail to incorporate neuroinflammation mechanisms into their design. Park et al. designed a triculture system utilizing neurons and astrocytes,  differentiated from progenitor cells, and microglia. This model exhibited key AD symptoms like Aβ aggregate and phospho Tau formation. Factors like CCL2 and ATP  are also produced and can draw in microglia from an outer chamber. Microglia then promote inflammation and reduce the overall neuron and astrocyte count. Park et al. are hoping this improved model system provides a more accurate representation of AD development, aiding in future potential therapies. BioLegend is committed to advancing AD research by providing reagents to study cells of the CNS and identify protein aggregates in neurodegenerative diseases. Adapted from Park, J. et al. 2018. Nat. Neurosci. 21:941

 Amyloid Beta Reagents

β-Amyloid, 1-15 Antibody (aggregate-preferring)

β-Amyloid, 1-16 Antibody

β-Amyloid, 1-42 Antibody

β-Amyloid, 17-24 Antibody

LEGEND MAX™ &beta-Amyloid x-40 ELISA Kit

LEGEND MAX™ &beta-Amyloid x-42 ELISA Kit

 

Purified β-Amyloid, 1-15 Antibody (clone 3A1)

IHC staining  clone 3A1 on frozen normal (left panel) and Alzheimer's disease (right panel) human brain tissues. 

  

 

Additional Neuroscience Markers

Glial Cell Marker Antibody Sampler Kit

CD11b

CD68

GFAP

MAP2

Tubulin Beta 3

 

Alexa Fluor® 594 anti-Tubulin β 3 (TUBB3) Antibody 

ICC staining of Alexa Fluor® 594 anti-Tubulin β 3 (TUBB3)
(clone TUJ1) on SH-SY5Y neuroblastoma cells.
Nuclei were counterstained with Hoechst 33342.