AAT Bioquest has developed the largest collection of calcium indicators, such as high-demand Fluo-8®, Cal-520®, and Calbryte™ probes, as well as calcium flux assays to track calcium mobilization with intense fluorescent signals and in a variety of wavelength options ranging from ultraviolet to infrared.
AAT Bioquest classic green as well as long-wavelength probes, including their unique NIR calcium indicator Cal-770™, are manufactured in-house for dependable supply.
Choosing the right probe depends mainly on the experiment. For assistance with product selection, recommended protocols, or other experimental concerns, contact our Sales Representatives.
Red-shifted calcium indicators are incredibly useful for researchers looking to perform multicolor experiments in combination with green- or blue-tagged probes.
Like Rhod-2, Rhod-5N AM is essentially nonfluorescent until binding Ca2+, then exhibits strong fluorescence enhancement with no spectral shift.
Ratiometric Calcium Indicators:
Performance Comparison: ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells measured with Fura-2 AM, Fura-8™ AM and Fura-10™ AM.
Upgraded Single-Wavelength Green Calcium Indicators:
These indicators exhibit significant calcium-dependent changes in fluorescence intensity at saturating calcium levels without shifting either their absorbance or emission wavelengths.
• Cal-520® AM has both high sensitivity and >100 times fluorescence enhancement when bound to calcium.
• Improved signal/background ratio and intracellular retention properties make Calbryte™ 520 AM the most robust indicator for evaluating GPCR and calcium channel targets
• Fluo-8® is a novel, high-performing green calcium indicator which incorporates the same fluorescein core as Fluo-3 and Fluo-4.
The majority of AAT Bioquest indicators are available as membrane-permeant AM esters, and membrane-impermeant salt derivatives and dextran conjugates. They also offer custom conjugations as well as stand-alone reagents for researchers with highly unique requirements.