Understanding RNA-Seq and rRNA Depletion

news March 19 2020

Learn how library preparation enables sequencing on the Illumina platform with Zymo Research’s bioinformatics team member and UCLA NGS instructor, Dr. Michael Weinstein. He will also explore the challenges stemming from the high abundance of ribosomal RNA sequences in nearly all sample types.

All NGS experiments utilizing the Illumina sequencing platform require the preparation of a sequencing library. In this video, learn why this process is necessary and how it enables sequencing on the Illumina platform through solid-phase or bridge PCR. Following that, we discuss some of the specific challenges with RNA-Seq library preparation related to the high abundance of ribosomal RNA sequences in nearly all sample types.

IN THE FOLLOWING VIDEO, LEARN ABOUT:

  • Library preparation
  • Understanding the role of adapters
  • Solid phase/bridge PCR within a flow cell
  • Pros and cons of different methods for rRNA depletion

View the video here!