One-Step gDNA Removal and cDNA Synthesis SuperMix

news October 24 2019

TransScript®-Uni One-Step gDNA Removal and cDNA Synthesis SuperMix

TransScript®-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature (42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds.

Highlights

  • Broad range reaction temperature (42°C -65°C ) .
  • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
  • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
  • cDNA up to 20 kb.

Applications

  • Multiple copy and low copy gene detection
  • GC-rich or complex secondary structure RNA template
  • cDNA library construction, primer extension, 3’ and 5’ RACE
Component AU311-02 AU311-03
TransScript® -Uni RT/RI Enzyme Mix 50 μl 100 μl
gDNA Remover 50 μl 100 μl
2×TS-Uni Reaction Mix 500 μl 1 ml
Random Primer(N9) (0.1 μg/μl) 50 μl 100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl) 50 μl 100 μl
RNase-free Water 500 μl 1 ml