The 8D3 monoclonal antibody reacts with mouse CD71 also known as transferrin receptor protein 1 (TfR1). CD71 is a 170-180 kDa type II homodimeric transmembrane glycoprotein which is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation and is required for iron import from transferrin into cells by endocytosis. CD71 is expressed on malignant cells at high levels and its expression correlates with cancer progression. This high expression on malignant cells along with CD71’s ability to internalize, and the necessity of iron for cancer cell proliferation make the transferrin receptor an attractive target to exploit for the delivery of drugs into malignant cells. The 8D3 antibody has been shown to deplete CD71+ cells in vivo.
This Antibody Is Useful For:
- in vivo depletion of CD71+ cells
- Immunohistochemistry (frozen)
- Western blot
As always Bio X Cell antibodies are specifically formulated for in vivo use and feature:
- 95% purity
- Ultra-low endotoxin levels
- Preservative, stabilizer, and carrier protein-free
Don’t forget to add the appropriate control antibody for 8D3 and the correctly matched dilution buffer.