TotalSeq™ and Comprehensive Multiomics Solutions

news February 16 2021

 

Multiomics has transformed traditional sequencing experiments by providing unparalleled protein and genetic analysis on a single-cell or bulk sequencing basis. BioLegend offers comprehensive and unique products, including antibodies, panels, and hashtag reagents to enable protein detection by sequencing. Explore the capabilities of TotalSeq™ reagents and see how they integrate seamlessly into existing workflows, enabling to simultaneously analyze protein and RNA expression on a single cell level.

TotalSeq™ oligo-conjugated reagents enable protein detection by sequencing and integrate seamlessly into existing single-cell transcriptomics, bulk sequencing, or single-cell genomics workflows. Choose your experimental goal below and discover BioLegend`s comprehensive solutions for single-cell and bulk multiomics.

Single-Cell RNA and Protein Detection – Compatible with TotalSeq™-A, TotalSeq™-B and TotalSeq™-C reagents

Technologies like CITE-seq and REAP-seq utilize oligonucleotide-conjugated antibodies to simultaneously detect surface proteins and RNA. For this, we offer multiple formats of BioLegend`s TotalSeq™ antibodies, panels, and hashtags to incorporate into single-cell transcriptomics workflows, including Drop-Seq and those offered by 10x Genomics.

Use our website search tools to discover the diverse TotalSeq™ reagents or contact us for help.

TotalSeq™-A
TotalSeq™-B
TotalSeq™-C
Hashtag Reagents

Bulk RNA Sequencing – Compatible with TotalSeq™-A reagents

Bulk RNA-sequencing is commonly used to analyze RNA expression from a population of cells in suspension. BioLegend`s BEN-seq workflow, developed in collaboration with Illumina, stains cells in suspension with TotalSeq™ oligo-conjugated antibodies prior to bulk RNA sequencing, enabling the simultaneous profiling of cell surface proteins and RNA expression.

Multiomics Analysis Software – For single-cell RNA and protein data analysis

Processing and analysis of single-cell data can be complicated and require advanced tools. To simplify the process, BioLegend offers Multiomics Analysis Software (MAS), a free cloud-based program for the analysis of multiomics data. Featuring an intuitive user interface with flow cytometry-like gating, the software is accessible to all users irrespective of their bioinformatics background. Access the software here and watch the video tutorial.

Custom Reagents

If you can’t find the reagents you’re looking for, contact us to discuss your project and let us know how we can support your multiomics work at info@nordicbiosite.com.

Uncovering SARS-CoV-2 Specific Cells and Biomarkers

TotalSeq™ reagents can be used to enable:

  • Multiomic analysis (Protein + RNA) of immune cells and lung alveolar lavage in COVID-19 patients (TotalSeq™-A or B in combination with 10x Genomics Single Cell Expression Solution (3’ assay)).
  • TCR/BCR repertoire profiling in COVID-19 patients for immunotherapy and vaccine development (TotalSeq™-C with 10x Genomics Immune Profiling Solution (5’ assay)).

The TotalSeq™-C Human Universal Cocktail, V1.0 was recently used for single-cell multiomic analysis in the paper by Su et al. and revealed unique immune cell subpopulations in COVID-19 patients. The TotalSeq™-C Human Universal Cocktail, V1.0 is available as a ready-to-use cocktail with 130 markers and 7 isotype control antibodies.

Do you want to learn more about TotalSeq? Check out our NEWS & ARTICLES here.

References: Su, Y. et al. Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19. 2020. Cell https://www.cell.com/cell/fulltext/S0092-8674(20)31444-6