Thyroid Hormones and Metabolism

news January 20 2020

Triiodothyronine (T3) and thyroxine (T4), are tyrosine-based thyroid hormones primarily responsible for the regulation of a number of developmental, metabolic, and neural activities critical for normal physiology. T3 affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. T4, the prohormone of T3, is the principal hormone produced by the thyroid gland and the major form found in the circulation. Though levels of circulating T3 are much lower than T4 levels, metabolically it is the most active hormone.

Arbor Assays ELISA Kits

The DetectX® Triiodothyronine (T3) ELISA Kit (K056-H1/H5) will measure total T3 in assessment of thyroid gland function or metabolic determination. This assay measures total T3 present in urine, media and extracted serum, plasma or dried fecal samples.

  • Range: 5,000 – 78.1 pg/mL
  • Sensitivity: 37.4 pg/mL
  • Sample: Urine, Tissue Culture Media, and Extracted Serum, Plasma and Fecal Material
  • Time to Answer: 2.5 hours
  • Assay Sample Volume: 100 μL

Triiodothyronine (T3) ELISA Kit ;Standard Curve

The DetectX®Thyroxine (T4) ELISA Kit (K050-H1/H5) will measure total T4 with precise quantitation in all species.  This assay measures total T4present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples with outstanding sensitivity in as little as a few μL of a sample.

  • Multi-Format: Standard Range of 20 – 0.63 ng/mL with 10 μL sample volume or 2,000 – 63 pg/mL with 100 μL sample volume
  • Sensitivity: 0.23 ng/mL for 10 μL sample volume and 55.3 pg/mL for 100 μL sample volume
  • Sample: Serum, Plasma, Dried Fecal Extracts, Urine and Tissue Culture Media

Thyroxine (T4) ELISA Kit Standard Curve