The Truth Lies in the Placenta

news January 07 2022

Latest News from Norgen Biotek

Extraction of RNA from FFPE Equine Placenta

Norgen Biotek recently sat down with Dr. Pouya Dini, an experienced researcher with a specialty focus in equine placenta and biotechnology of reproduction, to discuss his important research on the role of microRNAs during pregnancy and placental development in horses. Dr. Dini is working to discover novel biomarkers for gestational pathologies by analyzing the expression of RNA in equine placental samples. The future implications of this research are truly outstanding as it works to uncover more about placental development in horses, and in turn, humans. To read Dr. Dini’s story and learn more about his work click here.

Placental Exosomes Discovered in Urine

Urine samples are unquestionably easier to collect than FFPE samples and have recently been identified as a viable sample type for discovering placental exosomes in patients with gestational diabetes (Oostdam et al., 2020). Between 2000 and 2010, the CDC reported a 56% increase in the percentage of pregnant women with gestational diabetes, which can lead to preterm birth, macrosomia, and future diagnosis of type 1 or type 2 diabetes in the child.  To learn more about the future implications of placenta urinary exosomes read more here.

The Truth Lies in the Placenta

Dr. Pouya Dini’s research alongside Oostdam et al. creates a solid basis for future research to further explore these avenues of diagnosing gestational pathologies and diabetes. Check out the kits used for this research below.

FFPE RNA Purification Kit

 

  • Extract total RNA (including microRNA) from FFPE samples
  • No phenol extraction step
  • Includes DNase for optional on-column DNA removal
  • Isolated RNA is of the highest quality and integrity
  • Isolate a diversity of RNA species
  • Purified RNA is suitable for a variety of downstream applications,
  • including Small RNA Sequencing. Find out more information on
  • Norgen’s NGS services
  • Purification is based on spin column chromatography that uses
  • Norgen’s proprietary Silicon Carbide resin separation matrix

View product here.

Urine Exosome Purification and RNA Isolation Midi Kit

  • Purification and enrichment of intact urinary exosomes for functional studies
  • Bind and elute all RNA irrespective of size or GC content, without bias
  • Isolate all sizes of exosomal and extracellular vesicle RNA, including
  • microRNA​
  • Versatile urine input volume (2 mL to 10 mL)
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes are required
  • No precipitation reagents nor overnight incubation required
  • Compatible with urine from any species
  • Pure exosomes are purified and free-from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

View product here.

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