Recombinant SARS-CoV-2 Omicron (B.1.1.529) Variants

Omicron (Pango lineage B.1.1.529) was newly classified as a variant of concern (VOC) by WHO on 26 November 2021 due to concerns regarding potential immune escape, increased transmissibility, reduction in vaccine effectiveness, as well as increased risk for re-infections compared to the Delta variant.

Mutations in the spike glycoprotein of Omicron

Sino Biological is developing a series of recombinant Omicron Spike Proteins to support the study of Omicron variant. The Omicron variant is by far the most divergent variant characterised by a high number of mutations in the spike protein. Several mutations (e.g.: N501Y, P681H) have been identified in previous variants and are associated with increased transmissibility, immune escape, or other properties.

Please contact us for more detailed information.
• 30 amino acid changes: among which 15 are located in the RBD region
• Three deletions
• Three insertion in the spike protein NTD

Sino Biological is developing a series of recombinant Omicron Spike Proteins to support the study of Omicron variant.

Cat# Molecules Host Tag Mutants
40592-V08H121 RBD HEK293 C-His G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H
40592-V49H7 RBD HEK293 C-His-AVI G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H
40592-V49H7-B RBD HEK293 C-His-AVI G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H
40591-V08H41 S1 HEK293 C-His A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H
40589-V08H26 S-ECD-Trimer HEK293 C-His A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F
40589-V49H3-B S-ECD-Trimer HEK293 C-His-AVI A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F
40589-V08B33 S-ECD Insect C-His A67V, H69del, V70del, T95I, G142D, V143del, Y144del, Y145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F
40588-V07E34 N E.coli N-His P13L, E31del, R32del, S33del, R203K, G204R

 

Contact us for more information

SEC-MALS Validated SARS-CoV-2 Prefusion Trimeric Spike Variant Proteins

The spike protein resides on the surface of SARS-CoV-2 virions in a trimeric conformation (also known as the pre-fusion format). Evidence showed that pre-fusion Spike trimer is relatively unstable. It is essential to develop a stable pre-fusion spike protein trimer to support the development of vaccines, therapeutic antibodies, and diagnostic reagents. Sino Biological has successfully developed a series of highly stable trimeric spike proteins, covering multiple VOIs or VOCs, such as Alpha (B.1.1.7), Beta (B.1.351), Delta (B.1.617. 2), Kappa (B.1.617.1), etc.

Cat# WHO Label Product Name Mutations
40589-V08H10 Delta SARS-CoV-2 B.1.617.2 Spike S1+S2 trimer Protein (ECD, His tag) T19R, E156G, 157Del, 158Del, L452R, T478K, D614G, D950N, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants
40589-V08H11 Kappa SARS-CoV-2 B.1.617.1 Spike S1+S2 trimer Protein (ECD, His tag) G142D, E154K, L452R, E484Q, D614G, F817P, A892P, A899P, A942P, K986P, V987P, Q1070H, H1101D and furin cleavage site mutants
40589-V08H12 Alpha SARS-CoV-2 B.1.1.7 Spike S1+S2 trimer Protein (ECD, His tag) (69-70, 145 deletion), N501Y, A570D, D614G, T716I, F817P, A892P, A899P, A942P, S982A, K986P, V987P, D1118H and furin cleavage site mutants
40589-V08H13 Beta SARS-CoV-2 B.1.351 Spike S1+S2 trimer Protein (ECD, His tag) L18F, D80A, D215G, 241-243 deletion, R246I, K417N, E484K, N501Y, D614G, A701V, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants
40589-V08H8 SARS-CoV-2 Spike S1+S2 (D614G) trimer Protein (ECD, His tag) D614G, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants
40589-V08H9 SARS-CoV-2 B.1.618 Spike S1+S2 trimer Protein (ECD, His tag) H49Y, 145-146 deletion, E484K, D614G, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants

 

200+ Recombinant SARS-CoV-2 Variants

Mutations in: RBD, S1, S2, S1+S2 ECD, Nucleocapsid Various Fusion Tag: • His • mFc, hFc, rFc • AVI

 

Covering New Lineages:
• Alpha (B.1.1.7)
• Delta (B.1.617.2, AY.1, AY.2, AY.3)
• Iota (B.1.526)
• Mu (B.1.621)
• Beta (B.1.351, B.1.351.2, B.1.351.3)
• Epsilon (B.1.427/429)
• Kappa (B.1.617.1)
• Omicron (B.1.1.529)
• Gamma (P.1)
• Eta (B.1.525)
• Lambda (C.37)

 

Contact us for more information