Optibodies™ – A series of monoclonal antibodies for immunohistochemistry
Optibodies™ are a series of monoclonal antibodies for immunohistochemistry developed by Nordic BioSite. They were created to boost quality and create options for users of IHC biomarkers in clinical pathology.
High quality is our drive, our IHC experts have a high level of competence and we have our own R&D and production. Only antibodies that reach excellent results in testing protocol and appropriate control tissues are approved as part of the collection. An important aspect is being user-friendly, Optibodies™ works for different protocols and platforms with standard buffers. Optibodies™ get high scores in NordiQC assessments which is a confirmation of the high quality.
Would you be interested in testing out Optibodies™ in your own lab, please contact us at info@nordicbiosite.com and we would be happy to discuss the options with you. Protocols for IHC automation platforms are available on the Optibodies™ product page.
How antibodies are optimized into Optibodies™
The main points when optimizing an antibody are selecting the correct tissues, which are appropriately fixed and processed, and of course, selecting the correct primary antibody. Optimization of antibodies should always be performed with more than one antibody against the same tissue antigen to get results for comparison. Different pretreatment methods are important to test as well as fine-tuning antibody concentration and incubation times. From a clinical perspective, antibodies must be specific with a high affinity towards their epitopes. Of course, they also have to be flexible and reliable to use and have a good Lot-to-Lot-consistency.
” These are all our goals during the whole process of producing Optibodies™ “
Our top-performing Optibodies™
We want you to experience the high quality of our Optibodies™ and we want to make it easy for you. Check out the images and the protocols for our Pan Cytokeratin, EpCAM, and Napsin A, all CE/IVD marked. We have protocols for Ventana Benchmark Ultra and Dako Omnis if you want to test them in your own lab.
Pan Cytokeratin (BS5)
Pan Cytokeratin, Renal Clear Cell Carcinoma (RCC)
Cytokeratins are expressed in a wide variety of epithelial tissues and are of great importance for carcinoma diagnostic. The challenge is to stain all the 20 different cytokeratins optimally. BS5 clone meets this challenge as one of the best clones on the market. The main advantage of the clone is the monoclonality, providing robust performance independent of the tissue and between many protocols.
EpCAM (BS14)
EpCAM, Prostate Adenocarcinoma
Ep-CAM, is an epithelial marker and can be of great help in the differential diagnosis of malignancies in the peritoneal and pleural cavities. The advantage of the BS14 clone is its robustness and optimal performance on multiple IHC platforms, using standard high pH HIER buffer. The BS14 clone is a good alternative with a more flexible dilution range.
Napsin A (BS10)
Napsin A, Kidney
Napsin A is predominantly expressed in the lung and kidney. It is important in the differential diagnosis of lung adenocarcinoma vs. squamous cell carcinoma. The main advantage of BS10 is its excellent performance, being sensitive and specific as well easy to optimize.
If you are interested in more news related to Optibodies read articles here or visit our Science Hub
For questions regarding Optibodies™ do not hesitate to contact us at info@nordicbiosite.com and we would be happy to discuss the options with you.