Leucocyte Stabilisation in CSF Samples

news June 17 2019

Leucocytes that have infiltrated the central nervous system can be detected using flow cytometry with high sensitivity. Poor stability and low cell numbers mean that cerebrospinal fluid (CSF) samples often are not usable due to sample degradation and need to be recollected.

TransFix® from Cytomark stabilises malignant haematological cells in CSF, making it possible to determine rare leucocyte subsets via flow cytometric analysis. By stabilising CSF with TransFix, samples are more likely to maintain cell numbers, reducing the need to repeat costly and invasive lumbar punctures.

TransFix/EDTA CSF Sample Storage Tubes Features:

  • TransFix/EDTA CSF Sample Storage Tubes are specifically designed for the stabilisation of cells within CSF specimens for up to 72 hours.
  • Higher lymphocyte yield after 30 minutes compared to untreated samples (see case study 2 on our website).
  • Quality controlled against CSF substitute, including the markers CD14, CD4, CD8, CD19, CD20, Igκ, Igλ, CD56. (see CoAs for full details).
  • Markers stabilised include CD10, CD19, CD34, CD45, HLA-DR, and CD117 (see case study 3 and case study 4).
  • Light scatter and key antigen expression patterns are comparable to fresh samples (see case study 3 and case study 4).
  • Specific protocol for CSF sample preparation with TransFix (see IFU).
  • Recommended in guidelines published in the British Journal of Haematology (see case study 1 on our website), and referenced in multiple publications (see our bibliography)