Latest News from Meridian Life Science

news December 14 2021

Specimen-Specific Mixes for Liquid Biopsy

qPCR/RT-qPCR Master Mixes Formulated for Direct Detection from Blood, Saliva, Urine or Stool Samples

Blood, saliva, stool and urine are all sample types which can be used in liquid biopsy assays to screen for hundreds of different cancer types. However these clinical specimens contain a variety of inhibitors which can impact the efficiency of a qPCR/ RT-qPCR reaction. As a result, samples typically require processing prior to testing in order to remove these inhibitors and extract the DNA or RNA.

Meridian’s new inhibitor tolerant and specimen-specific qPCR mixes have been developed to overcome the challenges of PCR inhibition in blood, saliva, stool and urine samples, simplifying the development of liquid biopsy cancer testing. The formulations are glycerol-free, provided at 4x concentration, and can be air-dried to create ambient-temperature stable assays or kept in a liquid format.

Download flyer for more information

Simplified Workflow – No DNA/RNA Extraction Needed


New Air-Dryable™ RNA & DNA LAMP Mixes

Ready-to-use, 4x concentrated, fully optimized mixes designed to dramatically speed up assay development time and reduce TTR by 50%

Meridian’s new Air-Dryable™ DNA LAMP and RNA/DNA LAMP Mixes contain an optimized blend of enzymes, dNTPs, Mg2+, excipients, and stabilizers that are used with customer-selected primers and probes to create any custom DNA or RNA isothermal assay.

Experimental data on both RNA and DNA templates demonstrate that the mixes are up to 2x faster than other suppliers, generating results in half the time. In addition, the mixes incorporate Meridian’s new Air-Dryable™ technology and can be used in a liquid format or oven-dried to create an ambient-temperature stable assay (please see the product guide and FAQs for recommendations on oven drying parameters).

Product Art No Volume Reactions

Download flyer for more information

Air-Dryable™ DNA LAMP Mix MDX119 5 mL 800 Rxn
50 mL 8,000 Rxn
Air-Dryable™ RNA/DNA LAMP Mix MDX118 5 mL 800 Rxn
50 mL 8,000 Rxn

Air-Dryable™ RNA/DNA LAMP (MDX119) mix was air-dried with Dengue type 2 primers at 50°C for 80 mins. The sensitivity of air-dried Air-Dryable™ RNA/DNA LAMP mix (red) was compared to its liquid (blue) format and NEB WarmStart® (orange) in a serial dilution of the target RNA (Dengue type 2 crude lysate). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Error bars represent standard deviation over 4 technical replicates. The results illustrate that the speed of Air-Dryable™ RNA/DNA LAMP mix, is twice as fast as NEB WarmStart® in either the dry or liquid format.


Meridian’s Air-Dryable™ Direct qPCR mixes are 4x concentrated, inhibitor-tolerant, fully optimized master mixes designed to directly amplify DNA and RNA from crude whole blood, serum or plasma lysates. They maintain reaction efficacy and sensitivity in up to 20% crude samples with anticoagulants and can detect very low titers of viruses, bacteria and cell-free nucleic acids in singleplex or multiplex reactions.

Product Art No Volume Reactions

Download flyer for more information

Air-Dryable™ Direct DNA qPCR Blood MDX092 5 mL 1,000 Rxn
50 mL 10,000 Rxn
Air-Dryable™ Direct RNA/DNA qPCR Blood MDX121 5 mL 1,000 Rxn
50 mL 10,000 Rxn

 

Contact Us