Coronaviruses (CoVs) are enveloped non-segmented positive-sense RNA viruses infecting human and vertebrates. They are classified into four types (genus), α-CoV, β-CoV, γ-CoV and δ-CoV. They can infect the respiratory, gastrointestinal, hepatic and central nervous system of human and many wild animals. The family of Coronaviridae constantly circulates within the human population and mainly causes mild respiratory diseases.
Recently, a new severe acute respiratory syndrome β-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome called coronavirus human disease 2019 or COVID-19. Typical clinical symptoms of these patients are dry cough, fever, breathing difficulties, headache and pneumonia. Disease onset may result in progressive respiratory failure, heart tissue damage and even death.
Reagents for SARS-CoV-2/COVID-19 Research
As the world is struggling to contain the novel coronavirus (COVID-19) outbreak, healthcare infrastructure and testing capacity have emerged as major issues. There is an urgent need for access to accurate and standardized diagnostics for SARS-CoV-2 (the causative agent of COVID-19). Laboratory testing for COVID-19 and the associated SARS-CoV-2 virus includes methods that detect the presence of the virus (PCR technology) and those that detect antibodies produced in response to infection. Detection of antibodies (serology) can be used both for clinical purposes and population surveillance. AdipoGen Life Sciences has an ongoing pipeline for detection of soluble ACE2, antibodies against SARS-CoV-2 and a HTS detection kit for the determination of SARS-CoV-2 blocking reagents.
Detection of antibodies against SARS-CoV-2 in patients with COVID-19: Z. Du, et al.; J. Med. Virol. 92, 1735 (2020)
New Highly Specific SARS-CoV-2 Neutralizing Antibodies Detection Kit
ELISA PlateAdipoGen Life Sciences has developed a sensitive, specific and fast SARS-CoV-2 Neutralizing Antibodies Detection Kit (Prod. No. AG-48B-0002) that can be used to detect the presence of neutralizing antibodies against SARS-CoV-2 in serum/plasma in about 2 hours independently of species and isotypes!
This Detection Kit is based on a colorimetric reaction, which measures the binding of the RBD of the Spike S protein from SARS-CoV-2 to its human receptor ACE2. The presence of neutralizing/blocking antibodies in the samples are detected by reduction of signal indicating the inhibition of the Spike-ACE2 binding.
The SARS-CoV-2 Neutralizing Antibodies Detection Kit contains key reagents required to test the presence of functional neutralizing antibodies against SARS-CoV-2 present in the serum or plasma. It is an easy, fast and scalable (automation) alternative to the classical neutralization assays using Vero E6 cells, such as virus neutralization test (VNT) and pseudo-virus neutralization test (pVNT) and does not need biosafety requirements.
This kit is administrable in vaccine and therapeutic development as it is suitable for all antibody isotypes and can be used to determine neutralizing antibodies in animal models. The kit will also be useful in current COVID-19 investigations of sero-prevalence, assessment of immune response, herd immunity, longevity of protective immunity, efficacy of different vaccine candidates as well as tracking infections in animals.
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NEW Neutralizing Antibody Detection Kits for UK, BR and SA Variants
- SARS-CoV-2 Neutralizing Antibodies Detection Kit (B.1.1.7 Variant, UK)
- SARS-CoV-2 Neutralizing Antibodies Detection Kit (P.1 Variant, BR)
- SARS-CoV-2 Neutralizing Antibodies Detection Kit (B.1.351 Variant, SA)
- COMING SOON – SARS-CoV-2 Neutralizing Antibodies Detection Kit (B.1.617 Variant, IND)
New & Unique SARS-CoV-2 (Spike RBD) IgG Serological ELISA Kit
AdipoGen Life Sciences has developed a sensitive and specific SARS-CoV-2 (Spike RBD) IgG Serological ELISA Kit (Prod. No. AG-45B-0020) that can be used for the qualitative measurement of human Immunoglobulin G (IgG) against SARS-CoV-2 Spike (Receptor Binding Domain) protein in serum and plasma samples.
During an infection, IgM antibodies appear first, followed by IgA on mucosal surfaces or IgG in the serum. The spike (S) proteins (mainly the RBD domain) and the nucleocapsid (N) proteins are the main immunogens of coronaviruses leading to an antibody answer. This assay is an indirect ELISA assay (based on a coated Spike (RBD) protein).
Included in this assay and unique in the market for SARS-CoV-2 IgG assays, is a second plate (antigen non-coated Background Plate or False Positive Control Plate) to determine the sample specific background and to measure the amount of IgG antibodies non-specifically bound to the well. To measure presence of anti-SARS-CoV-2 human IgG antibodies in serum or plasma, net sample optical densities (Net OD) are calculated by subtracting each sample Background Plate OD from the Spike (SARS-CoV-2) antigen plate (Spike Plate) OD.
The Assay Kit can also be used with Dry Blood Samples, which is an inexpensive and easy alternative collecting blood!
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New SARS-CoV-2 Inhibitor Screening Kit
AdipoGen Life Sciences has developed a High Throughput Screening (HTS) detection assay for the determination of SARS-CoV-2 blocking reagents.
This assay is available as SARS-CoV-2 Inhibitor Screening Kit (Prod. No. AG-48B-0001), including all important and necessary components.
The SARS-CoV-2 Inhibitor Screening Kit contains key reagents required to facilitate identification of SARS-CoV-2 inhibitors. This inhibitor screen is based on a colorimetric ELISA kit, which measures the binding of the RBD of the Spike S protein from SARS-CoV-2 to its human receptor ACE2. This assay allows to identify and characterize the effect of different inhibitory molecules including antibodies or chemicals on the prevention of binding of SARS-CoV-2 virus to any ACE2-expressing cells. It allows the in-house coating of the plate with either the SARS S1 protein or the ACE2 protein, allowing the customer to decide which type of inhibitors he wants to screen for. All required components for both approaches are provided and in addition an ACE2 human blocking antibody is provided as a positive control as a reference.
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New ACE2 (human) ELISA Kit
AdipoGen Life Sciences has developed a sensitive and specific colorimetric sandwich ACE2 (human) ELISA Kit (Prod. No. AG-45B-0023) that can to be used for the in vitro quantitative determination of soluble human ACE2 in cell culture supernatants, serum, plasma and urine.
ACE2 has been identified as a key receptor on target cells for SARS-CoV infections in 2002. ACE2 functions as the entry receptor of the new SARS-CoV-2 coronavirus that emerged in China in 2019 and is the cause of the new disease COVID-19. Strong binding of the spike protein of SARS-CoV-2 to ACE2, along with proteolytic cleavage of ACE2 by transmembrane serine protease 2 (TMPRSS2), facilitates entry of the virus into cells, viral replication and cell-to-cell transmission. ACE2 can undergo an ADAM17 (a disintegrin and metalloproteinase 17)-mediated “shedding” from endothelial cells, resulting in the release of the ectodomain into the circulation. This soluble form may act as a competitive interceptor of SARS-CoV-2 and other coronaviruses by preventing binding of the viral particle to the surface-bound, full-length ACE2. A reliable ELISA Kit to determine soluble ACE2 in the circulation can be useful in therapeutic developmental studies.
Soluble ACE2 might also be used as biomarker of hypertension and cardiovascular diseases.
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NEW Active & Stable Trimeric SARS-CoV-2 Protein
AdipoGen Life Sciences’ SARS-CoV-2 Spike Protein (D614G) (Stable Trimer) (rec.) (His) (Prod. No. AG-40B-6003) is a full-length soluble SARS-CoV-2 Spike protein (aa 16-1213) including a foldon trimerization motif, mutated Furin recognition site (R682S, R685S) and two stabilizing mutations (K986P and V987P). This protein has a His-tag fused to its C-terminus. This protein binds to the human SARS-CoV-2 receptor ACE2 and it binds to anti-SARS-CoV-2 Spike antibodies in serum or plasma. It is useful for structural biology research, vaccine development, serological diagnostic kit development and neutralizing antibody screening.
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UNIQUE Human ACE2 Monoclonal Blocking Antibody
AdipoGen Life Sciences’ anti-ACE2 (human), mAb (blocking) (AC384) (preservative free) (Prod. No. AG-20A-0037PF) is a monoclonal antibody that recognizes human ACE2 and works specifically in ELISA, Western Blot and Functional Application. The antibody blocks the binding of human ACE2 to the Spike protein of SARS-Cov-2.
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Flow Cytometry-Competent Human ACE2 Monoclonal Antibodies
AdipoGen Life Sciences’ anti-ACE2 (human), mAb (AC18F) (Prod. No. AG-20A-0032) is a monoclonal antibody clone that recognizes human ACE2 and works specifically in Flow Cytometry (FACS). The antibody is available in different formats, unlabeled (#AG-20A-0032) and Biotin-labeled (#AG-20A-0032B), as well as labeled with the dyes ATTO488 (#AG-20A-0032TD) and ATTO647N (#AG-20A-0032TS). All variants are FACS-competent using the appropriate secondary reagents.
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