Introducing the Exclusive 2′,3′-cGAMP BioSTING Assay Kit

Are you conducting research in cell signaling, inflammation, immunology, or drug discovery? Consider the benefits of the exclusive BioSTING FRET assay that provides a real-time readout of CDN production in vitro, with high-throughput screening.

DetectX® 2’,3’-Cyclic GAMP STING-Based FRET Detection Kit is here!

Arbor Assays is excited to announce a new product kit available with its exclusive BioSTING sensor. STING, a protein that binds to 2’,3’-cGAMP directly, was chosen as the base for a patent pending biosensor, BioSTING, developed by Pollock et al. for in vivo and in vitro testing.

The DetectX® 2’,3’-cGAMP STING–Based FRET Detection Kits uses BioSTING to mimic small molecule interactions with STING in the cGAS-STING pathway and measure 2’,3’-cGAMP. This BioSTING protein binds to 2’,3’-cGAMP and allows for wide application use when screening for other STING agonists and antagonists. It provides real-time readout of CDN production in-vitro and allows for high-throughput screening. Its measurements are directly linked to CDN ligand binding and occupancy.

This kit is ideal for:

  • Researchers in academia who are studying cell signaling, inflammation, immunology, oncology etc
  • Industry professionals from R&D and pre-clinical pharmaceuticals doing drug discovery and screening for oncology, immunology
  • Clinical-stage biopharmaceutical companies focused on discovering, developing and commercializing drugs and therapeutics.

Researchers may use this for:

  • Exploring more scientific research about cGAS-STING cell signaling
  • Measuring levels of 2’3’-cGAMP and other cross reactants/ small molecule cyclic dinucleotides (CDNs)
  • Providing a real-time readout of CDN production in vitro
  • Identifying agonists and antagonists (activators and inhibitors)
  • For in-vitro high throughput screening.

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RESEARCH RESOURCES

  • Ishikawa, H., et al. (2008). STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling. https://www.nature.com/articles/nature07317
  • Wu, J., et al. (2012) Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. https://pubmed.ncbi.nlm.nih.gov/23258412/
  • Sun, L., et al. (2012) Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway, 20 Dec 2012, https://doi.org/10.1126/science.1232458
  • Zhou, W., et al. (2018) Structure of the Human cGas-DNA Complex Reveals Enhanced Control of Immune Surveillance. https://doi.org/10.1016/j.cell.2018.06.026
  • Zhang, C., et al. (2019) Structural basis of STING binding with and phosphorylation by TBK1, https://doi.org/10.1038/s41586-019-1000-2
  • Liu, S., et al. (2015) Phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF induces IRF3 activation. https://doi.org/10.1126/science.aaa2630
  • Oliveira Mann, C., et al. (2019) Modular Architecture of the STING C-Terminal Tail Allows Interferon and NF-κB Signaling Adaptation, https://doi.org/10.1016/j.celrep.2019.03.098
  • Cai, X., et al.(2014) The cGAS-cGAMP-STING Pathway of Cytosolic DNA Sensing and Signaling, https://doi.org/10.1016/j.molcel.2014.03.040
  • Shang, G., et al. (2019). Cryo-EM structures of STING reveal its mechanism of activation by cyclic GMP–AMP, https://doi.org/10.1038/s41586-019-0998-5
  • Burdette, D., et al. (2011) STING is a direct innate immune sensor of cyclic di-GMP, https://doi.org/10.1038/nature10429
  • Wang, C., et al. (2021)  Synthesis of All Possible Canonical (3′–5′-Linked) Cyclic Dinucleotides and Evaluation of Riboswitch Interactions and Immune-Stimulatory Effects, 21 Oct 2017, https://doi.org/10.1021/jacs.7b06141.
  • Pollock, A., et al. (2020) A STING-based biosensor affords broad cyclic dinucleotide detection within single living eukaryotic cells. https://doi.org/10.1038/s41467-020-17228-y