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F4/80 Antibody – Recombinant version

news February 16 2017

#Immunology

Anti-F4/80 [Cl:A3-1] antibody is the most widely publicized and referenced mouse macrophage and microglia marker making it a valuable tool for research.

Absolute Antibody, have sequenced the original hybridoma (rat anti-mouse F4/80 clone Cl:A3-1; Gordon and Austyn, 1981) and produced recombinant and chimeric anti-F4/80 clone Cl:A3-1 versions: allowing greater experimental flexibility and control for the end user.

They are the ONLY commercial manufacturer of recombinant F4/80 [Cl:A3-1] monoclonal antibody.
They are the ONLY commercial manufacturer of recombinant chimeric mouse anti-mouse F4/80 [CI:A3-1] monoclonal antibody: allowing experimental flexibility never allowed before.
They are the ONLY commercial manufacturer who offer recombinant rat anti-mouse and chimeric mouse anti-mouse F4/80 Clone CI:A3-1 antibodies with an effector-silent Fc domain (Fc SilentTM), allowing greater versatility and confidence for in vivo and in vitro applications.
There proprietary AbAb recombinant platform allows us to manufacture chimeric versions of the F4/80 [Cl:A3-1] antibody : allowing you to ‘species switch‘ the antibody’s Fc domain (ie. change the species of the Fc domain to rat, mouse, human etc.), whilst still retaining the antibody’s original species and antigen specificity and sensitivity.
They offer mouse, rat, human, hamster un-engineered (wild-type) Fc and Fc SilentTM domains.

F4/80 is a mature mouse cell surface glycoprotein and is a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. High level of expression is found on most tissue macrophages including the spleen; liver kupffer cells; microglia in the brain and skin Langerhans cells.

Absolute Antibody recombinant mouse anti-mouse and rat anti-mouse F4/80 Clone Cl:A3-1 is supplied in purified, azide free, and low endotoxin versions to allow for in vivo applications.

For labelled mouse and rat anti-F4/80 [Cl:A3-1] – contact our Technical Supportteam support@nordicbiosite.com.

If you cannot find F4/80 in the format you are looking for or would like to obtain a quote for bulk orders, please contact support@nordicbiosite.com.

Name	Art No	Application	Size
F4/80	Ab00106-2.0	Depletion, FC, IHC	200 μg
F4/80	Ab00106-2.3	Depletion, FC, IHC	200 μg
F4/80	Ab00106-8.4	Depletion, FC, IHC	200 μg
F4/80	Ab00106-6.1	Depletion, FC, IHC	200 μg
F4/80 recombinant version	Ab00106-8.1	FC, IHC	0.2 mg

In Figure 1 murine bone marrow-derived macrophages (BMDMs) were pre-blocked with rat anti-mouse CD16 & CD32 (clone FCR-4G8) and stained with non-recombinant anti-F4/80 [Cl:A3-1] conjugated to Alexa Fluor® 647 (AF647), all commercially available from competitors. In Figure 2 BMDMs were stained with recombinant anti-F4/80 [Cl:A3-1] or isotype control (anti-fluorescein [4-4-20 (enhanced)] IgG2b (Ab00102-8.1). In Figure 3 BMDMs were stained with Fc Silent™ recombinant anti-F4/80 [Cl:A3-1] (Ab00106-8.4) or isotype control (Fc Silent™ anti-fluorescein [4-4-20 (enhanced)] IgG2b (Ab00102-8.4). These were fluorescently labelled using the secondary antibody, goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially available from a competitor. Whilst in Figure 2 the highest fluorescence signal is seen with the recombinant anti-F4/80 IgG2 (the isotype of the original hybridoma-derived antibody), the isotype control IgG2b (Ab00102-8.1) shows considerable signal overlap, indicative of binding of the antibody to Fc-receptors. This illustrates the importance of isotype controls in such experiments when using conventional antibody formats particularly when Fc-blocking reagents are incompatible with the system used due to reactivity with the secondary antibody. The Fc silent™ format however overcomes this issue as seen in Figure 3, where the Fc silent™ recombinant anti-F4/80 (Ab00102-8.4) yields a strong and distinct signal, whilst the isotype control (Ab00102-8.4) shows no discernible difference to the background staining from the secondary antibody alone. Therefore, with Fc Silent™ reagents, no Fc-blocking products are required. [Data courtesy of Lewis Taylor.]