Extracting DNA from Plant Leaves & Seeds

How do you get DNA from a leaf? It seems like a simple task, but issues with breaking down tough plant cell walls, PCR inhibitors, and hazardous reagents can make extracting quality DNA from a leaf quite challenging.

Quick-DNA Plant/Seed Kits

The Quick-DNA Plant/Seed Kits are plant DNA isolation kits designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure of these plant DNA isolation kits is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with Zymo state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using Zymo-Spin technology included in these plant DNA isolation kits. The eluted DNA from these plant DNA isolation kits is ideal for downstream molecular-based applications including PCR, arrays, etc.

Zymo Research’s Solution for How to Extract DNA From Plant Leaves and Seeds

Zymo Research’s Quick-DNA Plant/Seed kits completely trivializes the biggest challenges with traditional CTAB extraction and other DNA extraction methods. There is no need for hazardous phenol or chloroform and many problematic secondary metabolites are removed in a single centrifugation step.

In a nutshell, the plant tissue is lysed by efficient mechanical disruption through bead beating, followed by a series of centrifugation steps using spin-columns to purify the DNA and remove downstream inhibitors (Figure 1). The resulting eluted DNA is ideal for many downstream molecular biology and sequencing applications. This simple process can be done in as little as 15 minutes – in conjunction with tissue grinding and bead bashing – on a variety of plant samples, including leaves, stems, buds, flowers, fruit, and seeds, giving you a straightforward path to high-quality DNA.

 

Figure 1. No more long incubations or precision pipetting. With Quick-DNA, just bead-beat and spin for high-quality purified DNA.

 

Quick-DNA Plant/Seed Miniprep Kit

Highlights

  • Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.

View Product

Quick-DNA Plant/Seed 96 Kit

  • Rapid, simple, high-throughput (96-well) method for DNA isolation from tough-to-lyse plant and seed samples.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.

View Product