Easy RT-PCR Master Mix with zero-step protocol: Volcano3G RT-PCR 2x Master Mix

Volcano3G RT-PCR master mix enables amplification of RNA or DNA target sequences with quick and easy zero-step PCR protocol. Volcano3G DNA polymerase is an extremely thermostable enzyme that has both reverse transcriptase and PCR activity. These two-functions from a single enzyme facilitate zero-step RT-PCRs directly from RNA templates as reverse transcription occurs at the same time with DNA amplification during the cycled PCR elongation step. The Volcano3G RT-PCR Probe 2x Master Mix has been successfully used for Corona virus (SARS-CoV-2, COVID-19) detections by several different researchers.

Improved Sensitivity

Volcano3G is the next generation of our Volcano2G with an improved performance. Volcano3G shows an improved detection of mRNA down to 500 total copies, which is clearly distinguishable from the No Template Control (NTC). The brighter fluorescent signal provided by the Volcano 3G Master Mix enables an easy and precise quantification. Volcano3G RT-PCR mix showed to be more sensitive than Volcano2G RT-PCR mix by 10 times. Below, artifical HPRT1 mRNA was detected down to 500 copies with Volcano3G RT-PCR 2x Master mix with a sensitive DNA binding dye with negligible polymerase inhibition.

HRM experiment

Volcano3G RT-PCR 2x Master mix with GreenDye is suitable for high resolution melting (HRM) experiments, allowing you to quickly identify unspecific amplicons and thus saves time and effort when optimizing PCR.

Applications

  • Rapid detection and identification of RNA & DNA targets
  • Reverse transcription qPCRs (RT-qPCRs)
  • Real-time PCR
  • qPCRs
  • Multiplex PCR
  • Probe-based PCR
  • Pathogen detection
  • End-point PCR
  • SELEX

Products

Volcano3G RT-PCR Mix is available in various formats with or without ROX or GreenDye. Volcano3G RT-PCR 2x Master Mix is a ready-to-use master mix. It is supplemented with GreenDye, a highly sensitive DNA binding dye with low polymerase inhibition and suitable for high resolution melting experiments. Volcano3G RT-PCR 2x Master Mix (w/o GreenDye) is optimized for probe based assays. These are also available with or without ROX reference.

View the products here!