PureDireX Virus Nucleic Acid Isolation Kit (Column Based)
The Virus Nucleic Acid Isolation Kit provides a fast, simple, and cost-effective method for the isolation of viral DNA/RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of virus-infected cell cultures. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for the nucleic acid to be easily bound by the glass fiber matrix of the column. Contaminants such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash Step. The phenol extraction and ethanol precipitation are not required, and the high-quality nucleic acid is eluted in the RNase-free elution buffer. The viral DNA/RNA isolated with the Total Nucleic Acid Isolation Kit (Virus) is suitable for a variety of routine applications, including the Real-time PCR/RT-PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The entire procedure can be completed within 15-20 minutes.
Sample: Up to 300 μl of the whole blood
Sample: Up to 200 μl of virus sample
Format: Reagent and mini spin column
Sample material: Serum, plasma, body fluids
Operation time: 20 minutes
Elution volume: 50 μl
Specification
- Isolates both viral RNA and DNA, allowing simultaneous detection of both types of virus
- Removes inhibitors that might interfere with downstream assays, ensuring greater assay specificity, sensitivity and reproducibility
- Prepares nucleic acid samples in only 20 minutes
- Yields a concentrated sample that is suitable for direct assay (no precipitation required)
- Universal viral nucleic acid purification system – One kit for both DNA and RNA viral purification, allowing simultaneous testing of both viral types
- Environment-friendly -Less infectious plastic waste due to the reduced number of hands-on steps
- Safety – No phenol/chloroform extractions
- Versatility – Spin and vacuum formats available
- Quality – RNA suitable for downstream applications
PureDireX Total RNA Isolation Kit (Column Based)
The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for the isolation of total RNA from the whole blood, mammalian cells, and bacterial cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure RNA is eluted with the REL Buffer without phenol extraction or alcohol precipitation. The RNA purified with the Total RNA Isolation Kit is suitable for a variety of routine applications, including the RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA selection. The entire procedure can be completed within 25-40 minutes.
Sample: Up to 300 μl of whole blood, 107 mammalian cells, and 109 bacterial cells
Yield: Up to 30 μg
Format: Reagent and spin column
Operation time: 25~40 minutes
Elution volume: 50~200 ul
Feature
- Delivering high-quality total RNA with the fast procedure
- Ready-to-use RNA for high performance in any downstream application
- Consistent RNA yield from the starting material with a small amount
