Covid-19 Analysis In Wastewater

Several research groups worldwide are screening wastewater to identify, quantify and analyse SARS-CoV-2. Analysis of wastewater -used water that goes through the drainage system to a treatment facility- is one way that researchers can track infectious diseases that are excreted in urine or faeces and predict Covid-19 outbreaks. Nordic BioSite offers several tools for SARS-CoV-2 screening in the wastewater: from sample collection to analysis.

Sample collection

DNA/RNA Shield

Inactivate SARS-Cov-2 while preserving your DNA and RNA at room temperature. Automation and DNA and/or RNA purification kit compatible.

RNA extraction

Total RNA Purification Kits including Stool and Soil kits

Extract high quality & purity total RNA (including miRNA) without phenol. From a wide variety of specimens (feces, soil, etc). Eliminates PCR inhibitors including humic acids. Suitable for downstream applications including qRT-PCR and NGS.

Spin-column and 96-well plate formats available.

Automated platforms: TECAN, Hamilton, KingFisher and Qiacube

Quick-RNA Fecal/Soil Microbe Microprep kit

Purifies total RNA (including small RNAs) from various samples in a simple, reliable, and rapid manner. Inhibitor removal and small elution volumes. Suitable for downstream applications including qRT-PCR and NGS. Spin-column format.

Quick-RNA Magbead

High-throughput, magnetic bead-based isolation of total RNA (including miRNAs). DNA/RNA Shield included. Suitable for sensitive downstream applications such as qRT-PCR and NGS.

96-well plate format available.

Automated platforms: TECAN, Hamilton, KingFisher

RT-PCR analysis

2019-nCoV TaqMan RT-PCR Kit

COVID-19 detection based on the assays and protocols developed by the CDC. The kit includes ready-to-use 2X One-Step RT-PCR Master Mix and a positive control (2019-nCoV RT-PCR Positive Control) which contains 2 nCoV nucleocapsid target gene RNA (N1,N2) and RNase P (internal control)

Inhibitor-Tolerant RT-qPCR Mix

Extraction-free assays from sputum, saliva and stool

Fast RT-qPCR Mix

Mix for multiplex assays on a broad range of sample types

Quick SARS-CoV-2 rRT-PCR Kit

The kit targets 3 different regions of the SARS-CoV-2 nucleocapsid (N) gene and a host-specific target region (human RNase P gene). It contains a ready-to-use master mix, a positive control (viral and human RNA), and is compatible with high-throughput workflows. CE-IVD certified

Contact us for more information!