A novel way to perform Western Blot

news October 04 2019

Mix OneStep Blocker, 1°Ab and 2° Ab with membrane together, that’s all.

OneStep Blocker is a blocking solution for Western blot analysis.

This OneStep Blocker buffer not only provides blocking, primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. It, therefore, serves as both blocker and enhancer in Western analysis. With the 3-in-1 step procedure, OneStep Blocker is a time and labor economic solution for the time consuming and laborious Western procedure.

Save and Reuse your Antibodies

OneStep Blocker can also be generally used applied as TWO-STEP BLOCKER. By simply using it as the BSA or other blocking agents, you may perform Western Blot in a conventional way.

The protein band(s) can be more intensive (a higher signal-to-noise ratio) when using the ONE-STEP BLOCKER as a TWO-STEP BLOCKER. As a result, the antibody amount used can be reduced.

Features of OneStep Blocker – Western blocking solution and a signal enhancer (Protein free)

  • 3 steps in one: Block the membrane and dilute 1o  Ab & 2o  Ab in one step.
  • Enhance antibody signal: It shows a two- to five-fold increase in signal intensity for most protein targets, enabling much less protein to be detected with the same substrate and method.
  • Enhance time-saving: It saves at least 2 hours in the antibody detection process during the Western Blot, with only one hour needed.
  • Universal antibody diluent: Ready-to-use dilution buffer for most of 1o  Ab & 2o  Ab.
  • No blocking step needed: Just immerse the membrane in the OneStep Blocker solution with your antibodies. That’s all.
  • Effective with any ECL: After the antibody detection process, the signal can be developed with both HRP (horseradish peroxidase) and AP (alkaline phosphatase) substrates.
  • Less hands-on steps: No 3 wash steps are required, meaning no need to transfer the membrane in&out of the container.
  • Compatible with PVDF & NC membrane: Regardless of the pore size, the OneStep Blocker minimizes the background from non-specific protein binding by antibodies.
  • Improve protein detection: Improve the binding process of target proteins, so that specific antibodies can bind more effectively.
  • Protein free: Reduces overall background and minimizes non-specific signals often seen with ECL detection.

Product Information

OneStep Blocker – Protein Free Western Blocking Solution and Signal Enhancer – GeneDireX – BS001-B500ML

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