Science Hub

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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Beginner’s Guide to Choosing Isotype Controls

Antibodies are widely used in research for a plethora of applications, such as:

  • Flow cytometry: to detect cell surface markers, intracellular proteins, and signaling molecules
  • Western blotting: to detect and assess the expression levels and post-translational modifications of native or heterologously expressed proteins
  • Immunohistochemistry (IHC): to detect the abundance and physical location of proteins in healthy vs. diseased tissue, for example, in pathology laboratories.

Whatever your application, you will notice that isotype information is always provided on the datasheet for the antibody you are using.

The Lowdown on Real-Time PCR – Part 2

Welcome to part 2 of our real-time PCR series. In part 1, we went through the basics of real-time PCR, its advantages over end-point PCR, a typical workflow, data output, and the choice of fluorescent labeling systems available.

In part 2, we take a look at the different quantification methods available, setup tips, primer design and quality control.

Discovery of the DNA Double Helix – 65 Years On

This month marks the 65th anniversary of James Watson and Francis Crick’s groundbreaking paper in Nature, where they elegantly described the double helical structure of DNA. To celebrate, we take a look at the men behind the discovery and give a brief account of their work.

The Lowdown on Real-Time PCR – Part 1

In case the name doesn’t give it away, real-time PCR is a PCR application that monitors DNA amplification in real time. This means that amplification is monitored during the PCR reaction, and not at the end of the reaction as with end-point PCR, where PCR products are typically analyzed post-run on agarose gels.

Skip Lengthy DNA Isolation with Direct PCR!

Since its dawn in the 1980s, PCR has become a mainstay in most clinical and biological research laboratories. While Real-Time PCR is invaluable in gene expression analysis and determination of gene copy number, end-point PCR remains the method of choice for many screening applications, such as genotyping, paternity testing, pathogen screening, and transgene detection, to name a few.

Microorganisms, Microbiomes & Microbiomics

Microorganisms, whether commensal, symbiotic, or pathogenic, are likely to impact almost every facet of human and animal health and their surrounding environments. Depending on whom you ask, microbiomes refer either to the genomes of all of the microorganisms that reside within environmental niches, or the microorganisms themselves. Microbiomics is the study of microbiomes.

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