Science Hub

Molecular Biology

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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The Typical RNAi Workflow in 5 Steps

Andrew Fire and Craig Mello won the Nobel Prize in Physiology or Medicine in 2006, just 8 years after their breakthrough discovery of RNA interference (RNAi) as a mechanism that can degrade mRNA in a sequence-specific manner (1).

Gene Silencing – RNAi or CRISPR?

No matter what area of research you are working in, at some point or another you may need to decipher the function of a completely unknown protein or better understand the mechanisms of a cellular pathway.

There are many ways of going about this, either by interfering at the protein level through the use of small molecule inhibitors or blocking antibodies, the genetic level by altering the gene sequence that encodes your protein of interest, or at the transcriptional level by interfering with the RNA transcript.

This piece looks at CRISPR and RNA interference (RNAi) – two commonly used methods to interrogate gene function – and compares the pros and cons of each approach within a lab research setting.

RNA Isolation in 7 Minutes – Go!

Total RNA is the starting point for a plethora of applications that can help to answer questions about the mechanisms of human and plant disease, gene regulation, embryonic development, transcriptional responses to treatments and stimuli, and more. Regardless of the application in hand – whether it be cDNA synthesis for reverse-transcription PCR, RNA-seq, northern blotting or otherwise, sufficient amounts of high-quality RNA will be a requisite.

Gene Expression Analysis: RNA-Seq or Real-Time PCR?

If you’ve been following our blog posts over the last few months, you’ll have noticed our series on RNA-seq and real-time PCR. Although the underlying principles behind these two techniques are different, both can provide information about the amount (absolute or relative) of mRNA isolated from any sample type.

RNA-Seq 5: Data Validation

RNA-seq generates vast amounts of data and when all of this is sorted out and boiled down to results, the researcher is usually left with lists and heat maps showing transcripts that are expressed, upregulated or downregulated across the experimental conditions tested. Exactly how and in what situations this data should be validated is a topic of debate among researchers.

RNA-Seq 4: Planning Your Experiment

Welcome back to the RNA-seq series! In this article, we will go through some of the main parameters you will need to consider when setting up a new RNA-seq experiment!