Science Hub

Immunology

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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Plex It up with LEGENDplex™ Multiplex Bead-Based Immunoassays!

Updated in April 2022.

LEGENDplex™ from BioLegend is a bead-based multiplex immunoassay that allows you to simultaneously detect and quantify up to 14 different analytes from panels that span hundreds of protein, chemokine, and cytokine targets across the fields of cardiovascular biology, extracellular matrix biology, immunology, metabolism and endocrinology, stem cell biology and toxicity.

Recombinant Antibodies – Part 2

In our last blog post, we introduced recombinant antibodies (rAbs), compared them to monoclonal antibodies (mAbs), and provided a brief overview of the rAb production process. In this follow-up post, we take a closer look at the rAb production workflow as well as the many applications for rAbs.

Recombinant Antibodies – Part 1

Recombinant antibodies (rAbs) are monoclonal antibodies that are generated in vitro using synthetic antibody-encoding genes. In contrast to monoclonal antibodies (mAbs), which are obtained through hybridoma technologies that require the use of animals, the rAb production process is completely animal-free.

In this blog post, we explain how rAbs differ from mAbs, and provide a brief overview of the rAb production process.

Control Your Flow From Start to Finish!

As with most other experimental workflows, controls are critical in flow cytometry. Several types of controls may be used in a typical flow cytometry workflow, and these collectively serve one major purpose: to allow you to confidently distinguish your findings from background variation and non-specific staining and leave you with meaningful data.

Within a clinical laboratory where flow cytometry is used to stain cells for diagnostics, e.g., cancer, immunological diseases and others, or treatment monitoring purposes, controls become even more important since the experimental outcome can inform a patient’s prognosis and play a major role in decisions regarding treatment.

Click Chemistry for Biologists!

The term “click chemistry” was coined in 1999 at the 217th American Chemical Society annual meeting by Karl Barry Sharpless, a renowned chemist and professor in the Department of Chemistry at Scripps Research Institute’s California Campus.

In a key review in 2001, Sharpless used the phrase again to describe chemical reactions that are simple and high yielding, wide in scope, create only by-products that can be removed without chromatography, are stereospecific, and can be conducted in easily removable or benign solvents (1).

To Gate or Not to Gate?

Flow cytometry is one of the most intensely used techniques in immunology that creates big amount of data in short amount of time. It is possible to obtain information on cell surface markers, cellular processes such as apoptosis, and signaling pathways and much more.

To get a reliable result in flow cytometry, proper controls for accurate gating is essential. Gating means to sequester specific group of cells. This is generally a manual step performed during and after data acquisition. Even though gating can be straightforward for substantial cell groups such as CD4+ cells in PMBC samples; it can be tricky when it comes to less obvious populations, for cells that have transient marker expression or small cell populations.

In this blogpost, we want to give some tips on how to use flow cytometry controls to get more successful gating and subsequently more consistent data.