RNA Extraction, Handling, and Storage

blog / Molecular Biology December 06 2022
pcr diagnostics kit

As promised in the last month’s blog we are back with chapter 2 of sample collection and handling. Here we focus on RNA extraction, handling, and storage.

RNA, unlike DNA, is highly prone to degradation; hence, one should take extreme care while handling samples for RNA analysis. While working with samples for RNA extraction we should be fast, clean, aseptic, and highly focused. In contrast to DNA, RNA is not forgiving and is prone to quick degradation. Hence all the above-mentioned precautionary conditions are a must. Make sure the following things are given a proper check and you are off to a good start.

  1. Ensure wearing all protective equipment including gloves, mask, and lab coats while handling samples and extracted RNA. This is to safeguard the samples from RNases.
  2. Keep a designated area for RNA works and ensure that no unwanted items are handled in the area.
  3. Clean the area prior to RNA works, to minimize the exposure to RNases
  4. Disposable RNase free plastic consumables. Click here to read about plastics and consumables offered by Nordic BioSite.
  5. Use of RNase inhibitors.
  6. Keep samples on ice whenever possible and store the sample in -80°C in a designated box.

View products that is suitable for your RNA sample preparation

RNA Extraction

Like in every research there is no ‘one-size fits all’-answer to RNA extraction. The method employed for RNA extraction varies depending on the samples being processed. Does it contain a high level of nucleases and other inhibitors that can potentially hinder the final quality and yield of RNA? How much sample is being processed and what is the desired yield?

View a wide spectrum of products for RNA extraction

RNA Handling

The most important thing while handling samples for RNA extraction and extracted RNA is the personal protective equipment together with plastics and consumables. Gloves and masks should be RNase free and single time use only. Thereby decreasing the exposure to external RNases. Yet another main source of RNases is the plastics and consumables used for the RNA study. Ensure that the plastic consumables including pipettes, pipette tips, tubes, and all other consumables that come in direct contact with the samples are decontaminated and RNase free. The eluted RNA can be stored in TE Buffer or RNase free water.

Discover a broad portfolio of available RNA handling products 

RNA Storage

After spending hours in the laboratory carefully isolating RNA it is very important to store them properly for downstream processing and analysis. RNA is usually stored in RNase free water or TE Buffer at -20C or at -80C for long term storage up to a year. It is highly recommended not to thaw RNA multiple times and prepare aliquots and store at the appropriate condition in the designated boxes.

View multiple products for RNA elution and storage 

We are constantly expanding our portfolio and you can reach out to us directly for any customized products that you are interested in and will be always happy to help you.

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